Why is hCG dosed in IU rather than mg?

Like all gonadotropins, hCG is calibrated against an international biological potency standard rather than by mass. One IU corresponds to approximately 1.68 μg of pure peptide. This convention is preserved in research protocols because it directly tracks bioactivity rather than weight, which can vary with glycosylation pattern.

How is hCG different from luteinizing hormone (LH)?

hCG and LH share the same alpha subunit and act on the same receptor (LHCGR). The unique beta subunit gives hCG additional carbohydrate chains and a much longer circulating half-life (~45 h vs minutes for LH), which is why hCG is preferred as a research tool for sustained receptor activation.

How should hCG be stored?

Keep the lyophilised glycoprotein at −20 °C in the freezer. After reconstitution, refrigerate at 2-8 °C and protect from light. Avoid vigorous shaking and repeated freeze-thaw cycles, which can damage the heavily glycosylated structure.

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