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Reconstitution calculator: concentration & aliquot volume
Vial amount + diluent volume + target research quantity → stock concentration, aliquot volume and the matching reading on a graduated U-100 volume scale. The maths runs locally; nothing leaves your browser.
Loading compounds… You can already enter values below as a custom compound.
Result
U-100 units are volume marks (1 unit = 0.01 mL), not International Units of biological activity. Edit the reading to back-solve the target quantity.
Parameters
Use the volume specified for your product or research protocol. The shortcuts are neutral volumes, not recommendations.
Enter the quantity specified by your research protocol. The slider spans 0 to the vial amount and always includes the value you type; changing the unit converts the value exactly.
Graduated volume scale
Enter a vial amount, diluent volume and target quantity to see the level on the scale.
All three share the U-100 scale (100 units = 1 mL). Smaller barrels have finer printed marks, so small volumes are easier to read.
Alternative diluent volumes
How the calculation works
Reconstitution dissolves a lyophilised (freeze-dried) vial in a measured volume of diluent. The relationships are deterministic:
Arithmetic example — a 10 mg vial in 4 mL with a 1 mg target quantity:
Exact vs. printed. The exact reading is the calculated value. The nearest printed mark is the closest graduation physically marked on the chosen scale; the two can differ, and this page always shows both rather than substituting one for the other. Multi-compound blends are calculated one component at a time, because a blend's total vial mass is not any single compound's concentration.
Further reading: the research glossary defines terms such as lyophilised, diluent and aliquot, and Peptide 101 is a plain-language introduction to research peptides.
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