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  • How-to guides

    Reconstitution calculator: concentration & aliquot volume

    Vial amount + diluent volume + target research quantity → stock concentration, aliquot volume and the matching reading on a graduated U-100 volume scale. The maths runs locally; nothing leaves your browser.

    Loading compounds… You can already enter values below as a custom compound.

    Result

    U-100 units are volume marks (1 unit = 0.01 mL), not International Units of biological activity. Edit the reading to back-solve the target quantity.

    Concentration
    – mg/mL
    Aliquot volume
    – mL
    Per scale unit
    – mcg
    Aliquots per vial
    –

    Parameters

    Use the volume specified for your product or research protocol. The shortcuts are neutral volumes, not recommendations.

    Enter the quantity specified by your research protocol. The slider spans 0 to the vial amount and always includes the value you type; changing the unit converts the value exactly.

    Graduated volume scale

    Enter a vial amount, diluent volume and target quantity to see the level on the scale.

    All three share the U-100 scale (100 units = 1 mL). Smaller barrels have finer printed marks, so small volumes are easier to read.

    Alternative diluent volumes

    Diluent Concentration Exact reading

    How the calculation works

    Reconstitution dissolves a lyophilised (freeze-dried) vial in a measured volume of diluent. The relationships are deterministic:

  • Concentration (mg/mL) = vial amount (mg) ÷ diluent volume (mL).
  • Aliquot volume (mL) = target quantity (mg) ÷ concentration (mg/mL).
  • Scale reading (U-100 units) = aliquot volume (mL) × 100.
  • Aliquots per vial = vial amount (mg) ÷ target quantity (mg), rounded down.
  • Arithmetic example — a 10 mg vial in 4 mL with a 1 mg target quantity:

  • Concentration = 10 ÷ 4 = 2.5 mg/mL
  • Aliquot volume = 1 ÷ 2.5 = 0.40 mL
  • Scale reading = 0.40 × 100 = 40 units
  • Aliquots per vial = 10 ÷ 1 = 10
  • Exact vs. printed. The exact reading is the calculated value. The nearest printed mark is the closest graduation physically marked on the chosen scale; the two can differ, and this page always shows both rather than substituting one for the other. Multi-compound blends are calculated one component at a time, because a blend's total vial mass is not any single compound's concentration.

    Further reading: the research glossary defines terms such as lyophilised, diluent and aliquot, and Peptide 101 is a plain-language introduction to research peptides.

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