Peptide Reconstitution Calculator

How the Calculation Works

The math is straightforward once you understand the relationship between mg, mL, and syringe units:

1. Concentration = Peptide in vial (mg) ÷ Water added (mL)
   5 mg ÷ 2 mL = 2.5 mg/mL
2. Volume to inject = Desired dose (mg) ÷ Concentration (mg/mL)
   0.25 mg ÷ 2.5 mg/mL = 0.1 mL
3. Syringe units = Volume (mL) × 100 (for a 100-unit syringe)
   0.1 mL × 100 = 10 units

A standard 1 mL insulin syringe has 100 unit markings. Each unit equals 0.01 mL. So if you need to inject 0.1 mL, you draw to the 10-unit mark.

Common Questions

• What if I use a 0.5 mL syringe? - A 50-unit syringe (0.5 mL) has the same unit-to-volume ratio: each unit is still 0.01 mL. The calculator results are the same; you just can't draw more than 50 units per injection.
• Does it matter how much water I add? - More water = lower concentration = more units per dose (larger injection volume). Less water = higher concentration = fewer units per dose. The total mg in the vial stays the same either way.
• mcg vs mg? - 1 mg = 1,000 mcg (micrograms). Many peptide protocols specify doses in mcg. Just divide by 1,000 to convert to mg for this calculator, or multiply the mg result by 1,000 to get mcg.